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CRIMIC CR72034

Gene NdufAF4

CG11722

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p1	III:10044596..10045542	85F7-85F7

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATAGCATAACGAACAGCTGATTCG	TCCTCCAGTGATAACAGCGTG

Images

Construct Design Sequences

sgRNA sequence	GAACTTGGGATCCACTGTAATGG
sgRNA2 sequence	TCTGCGGGTTAAATCGAGCTCGG

 

left_200bp	right_200bp
TTGGCATGGGTCAGGTGGTTTCAATGGTTGCCCGCCGGGCCAATCGCTTCAATGTCGAGAATCGTGCACATCGCGTCCTCGAGCGCGAGAAGCCAACGCCGGCGCCCAAATTTGACTCGAATTTGAGAGACATGGAGCGCACCTTGGAATGTAAGTAAAATTAAACACCCTTGGAGTGTAATGCTTCATTTTCCCCATTA	GCTCGGAGGGTAATCCGTAAATAAATCATTCTTGTTCTTTGTTTTAAACCTACGCTCTATTGTATGAAATCAAACAATGGCATTGGGCGGACAGTAGTATATGTTCAGGGACTTGTCCTCAGAAGCAGAGGCCACCTTGTCGTTTCCGGGGCTATATCGGACGCCCCACACCTGATCTGTGTGCTCGGCGAACGTGTGCA

 

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