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CRIMIC CR72036

Gene Egm

CG9006

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:11616869..11619023	48A3-48A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGAGCTTAATGTGCGCTGTCAA	TGCAAATCCAATTCCCAGTGCAGC

Images

Construct Design Sequences

sgRNA sequence	AAGTTTGTGCGAACCTATCGGGG
sgRNA2 sequence	AATAAACTTAACTACCAGCTTGG

 

left_200bp	right_200bp
TTTGTTTATTTGAATTTCTTGTAGACTATTTCAAAATCATAAAATTACCGTTACATACTTTAAATTTGTATGGCATGTCGATCAGCTGAGCAACGCCATAGGGTATTTTATAAAAGTACCGCTGGTAGGTTGTGCTTTATTTTATTCCATCTGCAGCTGGTCTCACTTGTAATCGCTATCGATAAGTTTGTGCGAACCTA	TGGTAGTTAAGTTTATTATTGCCTTTACGATTAAAGTGAATACTTGTTATTGATAATTCGCATGTGTTTAATCTCGTCTTGAGGGTTCCAAAAGGTAAAACACATTTGTGGATTATTTTCCGGGACACAATTTGACAACTAGTTTTTATATTTTATATTTCTAATAATTCACGCAAATATTAGCCAAAAAAAAAACTAAA

 

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