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CRIMIC CR72041

Gene Sdhaf3

CG14898

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:16481861..16482452	89B18-89B1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCTTGAAATCGCTTGGGGATTG	AGGAGCAGAACGAAGTGGAGAACA

Images

Construct Design Sequences

sgRNA sequence	ATACTATACTATTTAGCAGCCGG
sgRNA2 sequence	TTGCGCATCTTCTACTCCCTTGG

 

left_200bp	right_200bp
TTTGGTTTGCGGAAGTGTGGTTCAATTCGAAGTGCCTAACTTAAATATAATATGAAAATAGCATTGAACTTTTAAAGGTGACAATTCAAATGCGCGCCAAACTTTTAATACGTTGCCACCTGCCAACTCAATTTGACGTTAGCCAACACTGGCTGCGACAAAACAGTTGTTATTCGCTCGTATTTAGTTTTTAGCCGGCT	GAGTAGAAGATGCGCAAGGCAAATAAATCAGTGTATTATTTTATTATGAGTGTTTCTCCAAAATCTTGTACTCGAATTTAATGCCATTCTCCTCCTGCACACCCAGTGGCATGTCGGAATCGGGCGCGACCTCTCGAAAGCTGTCAGGGATCGCGGGGAAAAAGGTGTCGCAATCGAACTTCTGCATTATTTTGGTAATG

 

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