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CRIMIC CR72048

Gene Surf1

CG9943

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p0	III:6958346..6959586	65D4-65D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAAAGTATGATACGTTTGGGGAACC	CTTTCCCCATATCGACCCAAAC

Images

Construct Design Sequences

sgRNA sequence	ATGAGCTGGGGAATGGTTAATGG
sgRNA2 sequence	GAGGATACCCTTCTGAAAAAGGG

 

left_200bp	right_200bp
CGAAACCCGGGCACCGCGAACAATCCGAGATTAATCACCAGAAAGATGACACAACAGCGACCGCCGGTGAATTGGACAACCAGTATTCCCAATCAGGCGGCGAAGGACAAGGAGAAGATAGCTCCTTTGGGATGGTTCCTCCTGGTGGGTAGACACCAACTCCTCTACCTATACACACAACTAATCCTTGTTGTCCATTA	AAAGGGAACCCAAATCAAACGCACTGTTGTTATTTTTATTATAAAAATTTACATGCTCTCGTGTAAATGCTGGTTTTTATTGGCCCGCCGCAGGAGGGGATTATTGTCCTGCTGGGACTTAATATTGCAACTACCGATCCTCGAGTGGCGGATCGACAGGGGAATAGGAATAGGAATATGAATACAAATACGGTTCGAGG

 

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