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CRIMIC CR72049

Gene ND-B12

CG10320

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:21658078..21658777	57F6-57F6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCTCATCTCTAAGTCAACTGTGC	GGATCAGTCCATTACCAAATGCGG

Images

Construct Design Sequences

sgRNA sequence	ATTTCCAGTACAGCAATGGGCGG
sgRNA2 sequence	GGTTTATTCAGTATATCTAATGG

 

left_200bp	right_200bp
ATTACGGATATTTAAGCGGTTAATCAGTTAGTAAATAGCATTTAAATTATCTGTGTTCTTAGTAGATACAATTGGCTACTGCTAGCAGGCCTTGAAGCTTGTCAAACGTAAACATACTTATGTAATTGTTCAGGGCTAGGTTAAACATATGATTTTTAGCCTGGATAAAACTGATTTTTCCTTATTTCCAGTACAGCAAT	GATATACTGAATAAACCCATTGTGCTATCGCTCCAATTCACTTTATTCGCAATACATGTACGTGTAGGGGTTTTCACTAAAGCTAAAGTAGTCCTCCGATCAGTTAATGCGCGGCTTGAGCTCGTCGTAGCACGAGCGCAGAAAGATTTGGGGCTTCTTGGCCGGGTTGAAGTACTCGTGGCCCTTCGACCAGTCGTAGC

 

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