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CRIMIC CR72052

Gene dlt

CG32315

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:1789630..1795328	62B4-62B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGTGATGAAGTCATCACAACTCG	GATCGACAACCTCGTCCCCTTAT

Images

Construct Design Sequences

sgRNA sequence	GGGAGAGAGGGATTGGAGGAGGG
sgRNA2 sequence	GGCCGACTTATCGCGGGATCTGG

 

left_200bp	right_200bp
ATTTTACGTGTCGTTTACGTTGATTTAGTTTATTGTGTGAAAACATATTGTTTATACAGAAAAAAAAGAAAATCCTGCAACTTCAACCCATCCAAACAACTCTAAAAATAACTTAGAAAACGAAACCCACAAAAGCCGAGCCAACATTTCCCTTCTCTCCTCTTCCGTTGCAGGGAAACAAAAGGGAGAGAGGGATTGGA	ATCTGGATAACTTTTAAGTTTAAAACTGTTGGTTATGTCATTTAACGTTTATGTTTCGAATAAAGTGTTCCCTTTTTGTATGTGTCTGCTGGAAATGGAATGGAAACGGGGTCCACGCTATGTACCCGATAAGATAGCGTTAGCTGATGGAACCTAATCAGCGCGAGAATGCGTCACATTCGCGCTTTTAGTTTGGTTAG

 

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