CR72054

CRIMIC CR72054

Gene CG17036

CG17036

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	II:12698918..12700746	33F3-33F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CAGTGCTTTGGATATAAGTTCATATGTGC	CTGCTACTGCAATAGGCTACTTAGTAC

Images

Construct Design Sequences

sgRNA sequence	TGATTCGCGCGATCAGTTGCCGG
sgRNA2 sequence	ATGTGCAAGTTTGTAAATACAGG

left_200bp	right_200bp
TAGTTGTAGTAATCGTTGGAAATAATTTTATATGCCTGTCATATTGAATGTTGCTGTTTTCTTATGATTTTTTTTGAACCCCTTAAAATTCACCTGATTCTGGTTTTCCGGTTGTTGTGGGTGAATACTGCTGGCCACCACTGTAGCCGCTTTGGCGGAGAGCGATGGGCTACCAGGCGCTCTTGATTCGCGCGATCAGT	TACAGGACTTCTTATTTACTATCTTATTTATTCAAACAACATGGTATATAAGAAATTCCATCAATTAAACAAACTAGTACAAAGGTAAAAAGTTAAATCAGTTGTTGAGCAAAGAATTTCCTTTCCATAAAGTTAAACTTTACAGAAATAAAACATAAATGAAAGTCTAAGCTACTATTAAATTGAAAGGCTTTTAATAT