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CRIMIC CR72057

Gene Scsalpha2

CG6255

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:19461879..19463066	92A5-92A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCAGGCACAGTGGGGTAGATAAT	CACCTAGTTTTGGGAGCTACACC

Images

Construct Design Sequences

sgRNA sequence	GGAATTAAGTCTTGGATTGCTGG
sgRNA2 sequence	AGGCCGGCAAGAAGTAGGAGTGG

 

left_200bp	right_200bp
TAAAAGAAAATGCACTTAAACATGCATGTGCGTAAATTACTAGAAAGGGATTTGTTAAGAACACAGCAGGCCTTTTTCCAAACCTCCCTGATAATTTGACTGTTGAAACCTGACATTCGGTCAACAGTTTTGGTAAGAACTCCATTCTAAATCAGGCCCCAAAAGTTTAATTTTTTGGCCCAAAAACAAATCCACCAGCA	GAGTGGCGGAACAAGTTATTGATGCCAATAAACTAACTTAGTGCTCTTTAAATTGCTAAAATATCTCTTTTATTTTTTTTTTGAAGTCTAAATTATAAAATTGATGAAGAAGTTCAATGGGATTATATCTAAACACATAACAAACAGAAAAAATATATTTTTTTAATATTTAAGTAATGTTTAAGTAGTGTTTTGATAAA

 

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