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CRIMIC CR72063

Gene IleRS-m

CG5414

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:16100280..16103482	72D6-72D6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGATCAGCAATTCCACCAACCA	AGGATGGCTTCTCCAACATCAAG

Images

Construct Design Sequences

sgRNA sequence	ACGTTGTGTCTCTTCTTATTCGG
sgRNA2 sequence	ACGTTGCTCTGACGTTATGGAGG

 

left_200bp	right_200bp
TTAATTTGTATTTATTTTGAATTTACATTGTTATATTCAATTTATATTAAAGTAGTTACAAAATAAAATAATATCAATTCAAATTACATTTTAAACTTACAATCTACATCGCGGGCTGTGCACTGTGTGAACAGTGCTTACGTTCCACAAGACCATATGATTTCCACTTTGCCGGCTGTTGTTACGTTGTGTCTCTTCTT	TGGAGGCAAAGAACTAAGCGTAATGATGTGTTGTTATTTAGGTGTAAGGAATACATTTATGTTTTTAAAAACTCTGTAAGCATTTCATGATGGTGTCTCGCAGCTTGAGCCGGGTTGTTGGCTTTCCGCCGCAGATAATATGCGTCTCGTTGTCCTCGAACTTAATGACTGTGTCCTCATTGACAAGGTTCAGAGTCAGA

 

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