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CRIMIC CR72064

Gene IleRS

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:22288665..22292810	9D4-79D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCAGAAATTCAACTGACTTGCGG
sgRNA2 sequence	AGCTCCGTAAGCTTAACATAAGG

 

left_200bp	right_200bp
CTTATGGCTAACAAATTTATTAAAGTTGTTAATAGTAAATAAGCCTTTCTTTTTTTTTCAGTGCTGTCGTCAGCTGATTATCAAGGATGCCGCTCATCGGAACCTACTATCGATATCGCGAACCCATGAGCTAGAGAGCGATTAATGAACCACTAGTTCCGTACCTCGGGCACGTTGTGTGCCACGCAAAAACGCCGCAA	GTTAAGCTTACGGAGCTATTGGTATTTAATTGAAAACGAATCGAAACTGAAATAAATGTTGTCAACTTTTCAAAAAAGTTTTAAACCTTATGCAGCTTTGTTGATTTTGATTTTTTCCCGGGCTTTAGTATTTCTTTAATATGGTAGAATTACAAATTTAAAAGTATAGTAATGCATATTAGGTTCTAATATAGAGTTAC

 

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