CR72069

CRIMIC CR72069

Gene Maf1

CG40196

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_p2	II:1512810..1527095	41A1-41A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACTTGATAAGACACAATTCTGCGTAA	GAAGAAGGTGAAGGTAGTGAAGAGTCC

Images

Construct Design Sequences

sgRNA sequence	TCATCTTCATTATAGCAAAATGG
sgRNA2 sequence	TTAAGGGGTCAGAGTGAGCGTGG

left_200bp	right_200bp
ACAACAGACTTAAATTTATATGTATCATTTAAATTTAATTTATAAATCATGAAGTTACTTGAAAGTTCACGTTTTGAGGCTATCAACAACGCTCTTTCTATACAAACAAGTGGCATCACGATTTTTGGACGGATAGAAAGTTACTCATGGTATGTTTTTTTAGTTACATAGAAATATTTTTTAATTCATCTTCATTATAG	GCGTGGAATTTTTTTTGGCTTATCGATAACAGTTTAAACTAATACAACCATCAAAAAATATCAGTGTGCGCGTGGCAGTTTTGGGCGGTTGGTGGGCAGAAGAGTGCGAGTGGCAGCATGCGTCTATTTCCCTAGAATCTGTATGCTTAATCTTAACCTTGAAGCTTTTAAAGTTTATGAGATCTCGTCGTTCATCCTGA