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CRIMIC CR72095

Gene Epg5

CG14299

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_PM37_kG4	III:18750436..18758873	91C6-91C6

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGCAGCGTGAGTCACGACTGTTA	TGTTATCGTTCGTGTAGCCCTACG

Images

Construct Design Sequences

sgRNA sequence	GTTGTACGTTTGATTGCTTAGGG
sgRNA2 sequence	tatatcatctaactaggtatagg

 

left_200bp	right_200bp
tgtttaggtattttaccaagcattgtatttgacataaacatgCattttaaagcagctttatttactcgtgagccatcgtgactgctcccagctgttctccggtttctcccatcactattatatttatgggtctttttgtttggcgtttttgcggccccccaaagaatgcgaaaataaaacgcaaaaagtctgatccctaa	tataggttaaataaagtgataaattttaagcaattttcaaaattgcataaaatgtataaaagtgaacatttatttatacatactttttagcataatacaaacatggggTcaaacactgaaactttgttttaaatcttgtagactataactttatttgatatacatCtctcgaaagtgcatacttgaagctatttgtttaa

 

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