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CRIMIC CR80001

Gene eIF4G1

CG10811

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT_p0	4:915297..930715

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCTGAATGACAACTTGATATACACCGTT	ACAACAGATCACAAGAGTGGGAAC

Images

Construct Design Sequences

sgRNA sequence	TATGGACAAATAATGTTTATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TTTTAAGGCTAATTGTCAACATGCCGGCGACGACTAAGGAGCAACAGTCGGAAAATTAAATGAAGCTATAACAAAATAAATAATAGTTTAATTGTTTTTAATCATTTAGTGTTCTTATTTTGTACGCACAACTTTTTTTTTATTAATTAATTAATCTTGTAATCTATCATTTGAACTGTGTTAGACAGCTAAATCCCATA	GCAGGGAAACCTATTTGCACTACTAAAATCCCCACAATAAAACCCACCATCCCCCAGCTAATGGCCTGCATCAGCAAGATGTTGTATGCTCTAGGAATCGGCAGGCGCAATCGCGATCCCCATTACGATCGGTACCCCACACCCATGCGGCCACTGTGGCGGCGGCGGCAGCGGCGCTCGCCAGGGGGCGCAACCGCGAC

 

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