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CRIMIC CR80002

Gene Nna1

CG44533

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT_p1	X:13692292..13717835	12B3-12B4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTTGGGTCACTAGTTGGAGCG	CCATATTCTTTTCATCCTTGCCACC

Images

Construct Design Sequences

sgRNA sequence	TAGAAAGAGCTTTCACTGCAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
CAAATAAATGGGTTGCTTTTTAAACGGAATCAAAGATTGTAGATTCAAAGTCGCTTCAACTTAATGAACAAGAGGTTTTCTCTAGTTCCAGAAAGTGTAAGCTTTTTTGAAGGGACAATTTGAGGAAATATTACTTCATATCATGCAACTAAGTAAGGAAAATTGAAGATTCCTAAGATTCCCTAGAAAGAGCTTTCACT	GCAGGGAAACCTATTTGCACTACTAAAATCCCCACAATAAAACCCACCATCCCCCAGCTAATGGCCTGCATCAGCAAGATGTTGTATGCTCTAGGAATCGGCAGGCGCAATCGCGATCCCCATTACGATCGGTACCCCACACCCATGCGGCCACTGTGGCGGCGGCGGCAGCGGCGCTCGCCAGGGGGCGCAACCGCGAC

 

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