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CRIMIC CR80003

Gene Exn

CG42665

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT_p1	3L:16970013..16994388	73E1-73E1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAACCCATTTACCAAAACGAAGCG	GCTGTCTGTCTCAAAGGAGTCG

Images

Construct Design Sequences

sgRNA sequence	CAGCAGTTAGTTCTGTTTCAGGG
sgRNA2 sequence

 

left_200bp	right_200bp
TACAGCCACCAACGAGCCCAACTACTGACCTCCATGCGAGTGACAGTAGCGGAGGGAGCCGAAAATGACGCAAAGGTCACCATGGAAGTGAGCACATTCGTAGGTTCGTCCTGGTTTCATAAATAGATTATAGGAAAATAGATTAAGAATTTGGTAATAGGTGAAAATCAAGCAGAATCTTTCAGCAAATTCATCCCTGA	AACAGAACTAACTGCTGTCCATTCTGTAACTTTTCAGATGACTCAGACCACTACTACGAGACCCTGTCGCCACTCGGAAACAAACGCCATTCAACACAGCTGGTGCGGCAGAGCCAGGATGGAAATCGGAGCGGAGGGGTGCGCTCCTCGCACCGCCGGAGCAAAAACCACTCGGCATCCGCCCACACATTGGGCGAGCA

 

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