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CRIMIC CR80005

Gene Myo28B1

CG6976

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT_p0	2L:7666047..7689459	28C1-28C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTTGTGCGGCAACATGTAAGTG	GCAATGTATTCTACCTTAAGTAGTTTCG

Images

Construct Design Sequences

sgRNA sequence	GTAGTAATACCTGTGATTATGGG
sgRNA2 sequence

 

left_200bp	right_200bp
GTCGTGGCAGTTTTTCCCATCCATTCGCTCTGGTTATCAGAAAATTACTATGATAACCGAATCGTTTTATCACATTCCTTTGGCAGAGCCTCTGCTTTAGGCACATCATGGGTCTCATTTTAATTTTAAACTTCCCTCCTCTTATCAACTACGCTCACCTACACGTGGGGTGTTAATTGGAATGTAGTAATACCTGTGAT	TATGGGCATAAAGTATTAACGAGCAGGTAGCCTAACAAAAGATTGTGTCCGTCACGAGCTCAAATATGTCTCGGGTTTCATTTGCAGAATACAACCAAAAATAGCACTATATAAATTCCTTATACTTCCTCTCTGAGCAAATGTCTTATCAAAATCGAAGCCAATTATCGATTGAGCTTAGTCGCCACAGGAACAGAGAT

 

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