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CRIMIC CR80006

Gene Stlk

CG40293

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT_p1	2R:4546691..4565281

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTTAGCCACAGATTCCCAGACTG	TCAGCTGGTTGATGGGTGC

Images

Construct Design Sequences

sgRNA sequence	CTTGGAGGTCTATTGGGCAGCGG
sgRNA2 sequence

 

left_200bp	right_200bp
AAAGCAGAGCGAAAGCAGTTGCTCTAATTGATGGTGTCAAAAAATTGCATAATTGCTAAATTCCAAATTGTTTAGCATTTAACAATTTACGCATTTAACAATGCTACGTCGCTCTATATTCCTTAACGCATATAACTTCGATATATTGTAGTTTACCGCAAGGGATCCAAACTTGGGAATGCTGAGTTTCTTAGCCGCTG	CCCAATAGACCTCCAAGAGCGACGTCATCCATTTTAGAAGATTTACATTCAGCTCACCAGAGATTGGTAAAAATGAAGGCCCTAGCACCCAGCTAAGTTTGGTGACCCAACATCGATGCTACAATGAGTCAACTGTAAAAAACTGGAAATAGCATGCCAGGAAATCATGAATCATGAATCAAACGCCATTTTGACCAACT

 

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