| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | III:24485428..24491147 | 96A3-96A3 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | no band | L-F GTTGTGCGATTGTGTGCGG L-R caagcggcgactgagatgtc | R-F ggcatggatgagctgtacaag R-R GCTGTTGGATACACATGCTTGG |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|