| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | II:24047411..24053035 | 60B5-60B6 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F GTAGAGTATGTGTAACGAACAAGATCG L-R gtggtgcagatgaacttcagg | R-F acctcgatatacagaccgataaaacac R-R TGATGACAATTGGATCGGAGTTGATG |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|