| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | II:18599755..18605249 | 36F7-36F7 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F GGTCGTAGAAGTGGTTGTGG L-R atccagctccaccaggatg | R-F acctcgatatacagaccgataaaacac R-R TGCTGCTGGTAAGGAAACG |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|