| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | X:8070244..8075466 | 7D5-7D5 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | long band | L-F AGATGTACAACTACAAGCTGGTGG L-R cgactgagatgtcctaaatgcac | R-R GTTGGTGTGTCTCTTGTGTTTGGG R-F ggcatggatgagctgtacaag |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|