| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | :1103254..1108373 | 21F1-21F1 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | no band | L_F CTTGCGTGAAACGTGTGCC L_R GATAATCATGCGTCATTTTGACTCACG | R_R ACTCCAGACTCTAGAATAGATCCATCC R_F GGCATGGATGAGCTGTACAAGG R_R 2 CTCGGGATTCGCTATCACCTGTTT |
 |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|