| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | III:2252744..2257423 | 62D4-62D4 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F tgcagtatgtggccggatttg L-R gtgcagatgaacttcagggtcag | R-R GAAGGAGAGCTCGAGGAAAATG R-F acgaagcgcctctatttatactcc |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|