| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | :17633449..17638001 | 74E3-74E4 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | CR80039 L-F 2 ACCAACAATCTGGTGGCTCTACTG CR80039 L-R GAACCTCCCACCAGCACATAG | CR80039 R-F ACGATTTCGTTTTAGCGCAC CR80039 R-R ACGATTTCGTTTTAGCGCAC |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|