| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | 4:1060969..1077273 | 102F7-102F |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F GCATTATCGGTGTGCGAAGTG L-R CCCTTGGACACCATGGATC | R-F AACACATGCGTCAATTTTACGC R-R GCAAGTGGATCGTTAAACGAGTAAGAG R-R 2 AGCAGAACATCAACCCGCTAGCTT |
 |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|