| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | III:28820221..28824508 | 98E5-98E5 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| no band | OK | L-F cctatacctgaaaacccctccc L-R gtgcagatgaacttcagggtcag | R-R CCACGATTACGTATATGTATGTTGTCG R-F gagggttcgaaatcgataagcttg |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|