| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | :24101600..24105763 | 95E1-95E1 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F 2 GCGTTTACACGTGTGGTTTATGTG L-R TGAACAGCTCCTCGCCCTTG | R-R 2 GAGTGGAGTAGTGTTGTTCCTTTC R-F ACGAAGCGCCTCTATTTATACTCC |
 |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|