| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | :4103564..4107116 | 64A6-64A6 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F 2 CATCAGCATCAATTTGAAGGGC L-R CTTGGACACCATGGATCCGC | R-R 2 CTTCTGGTGTCGGACACTAGTC R-F GGGTTCGAAATCGATAAGCTTGG |
 |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|