| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | :8412144..8415256 | 29C3-29C3 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| OK | OK | L-F TTGCAGTAAACATTTGCAGAAACG CR80052_GFP_R gcccttggacaccatggat | R-R ATCCTGTTTGATCCCATGTGTGGT dsRed_F cagaccgataaaacacatgcgtc |
 |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|