| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| gRNA_int200_GFP_exon | II:10155916..10158931 | 46F3-46F3 |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| no band | OK | L-F GCACTTTGGGTTTCTTTGTTACG L-R gtgcagatgaacttcagggtcag | R-R GTGTGTGGCTTAGGTTAGGCAG R-F acgaagcgcctctatttatactcc |
| sgRNA sequence | |
| sgRNA2 sequence |
| left_200bp | right_200bp |
|---|---|