CR80088

CRIMIC CR80088

Gene Cdk8

CG10572

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT	III:9837080..9838670	67C10-67C1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	L-F GCAGGATCAGTACTTCCAGGAG L-R GCCCTTGGACACCATGGAT	R-R CTACTAGAGCTGGCAACACAGC R-F CAGACCGATAAAACACATGCGTC

Images

Construct Design Sequences

sgRNA sequence	GAGTGTATGGAGGCTACACGAGG
sgRNA2 sequence	GGAATTGTTGGTGTTTGATTAGG

left_200bp	right_200bp
GCCAGTGCGTCAGCCACGTCCCGTGACCGAGAAGCTGCCCGCCAACCACCCCCTGCTCACCGGACAGCGTGTGCTCGACTCGCTCTTCCCCTGTGTCCAGGGCGGTACCACCGCCATTCCCGGAGCTTTCGGTTGCGGCAAGACTGTGATCTCGCAGGTGAGAGTCCCACAAATTGAGAATTTAAGGAGCGATGCCTCGT	CAAACACCAACAATTCCGTGCTGCATTCGATGAAAAACAAAAGTCCAACAAATACCACAACTTCTTGGTGCCTGCGAGAGATGTAAACATTCCGGCCTGCGGTTAATACTTTCCCCTAACCACGCCCCCTCCGCCCCTTGAAGGGCAACTCTAGGCAACAGCAACTACAACGTCCTGCTATGTACTTCCATTTACAACAA