CR80090

CRIMIC CR80090

Gene apoltp

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_exon	2L:9634179..9656026	30C2-30C2

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CR80090 L-F CTTCGATCAGCGTTATGTGGGTC CR80090 L-R CCCTGAACCTGAAACATAAAATGAATGC	CR80090 R-R TGCACGAGAGCAGGATGAAG CR80090 R-F CTGTGCAGCAGCTTTAGCTTAC

Images

Construct Design Sequences

sgRNA sequence	CTGGAAAAGATCGGTTTATATGG
sgRNA2 sequence	GGATTGGGAAACGTTCATGCAGG

left_200bp	right_200bp
CACGATTTCTTCAAACGAAACTTCACTTTGCTTTTGGAACCAGCGGTAGGTATATAGGGAATATACCTTGTTTACTTAAGTAAATATATTTCTTTCAGAGCAAATCCCTGGCTGGCCAAGGACTAACACGCAAGTTGAGCTTTATTGCGAATGGTCAACTGATTGAAATCGACCTCGAAACAGATGTGAGTTAAGCATAT	TGAACGTTTCCCAATCCTTAAAACCAACCAGGTACGCTGTGGTGGCCTTCGGAGGAGTTTCTCCATATGACAAAGCCAGGAGTGTCATATACGAACACAACGAATTCACTTCCAAGCCGGAACAGCTGGCGGACTACTTTGGTCACATTAACACCGGAAATGGCAGCAGCAACGATATCCTAATGGCCATTTCCGCGGCT