CR80092

CRIMIC CR80092

Gene CG43689

CG43689

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_PT_p1	III:4075743..4092237	4A4-4A5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	L-F CATTCAGCAGCCAAAAAGACATTAAGC L-R gtgcagatgaacttcagggtcag	R-R GCAGCGAGCTTTAGAATCTACGG R-F acgaagcgcctctatttatactcc

Images

Construct Design Sequences

sgRNA sequence	ATGAGGATGTATGTAAGGGTGGG
sgRNA2 sequence

left_200bp	right_200bp
AGGGGGTGTGTGCTGTACAATTCGGTGTAGTTGGGGGTTTCGAGCGCACAGCAACTAATGGCTTGTAAACCCTTTATGCCACACAACACAGAGGCACATGGACAGACATCTTTTGGTTGAGTGCCTGAGCCTTGAGCTTTGCCTTCTACTAATTTCGCAGTACAATTTCCACAGGCGACGACGATGAGGATGTATGTAAG	GGTGGGCGGGGTAAGTGGGTGTGGCATGATTAGATATAAAGCATTATGAGCACGTTTTCCTTCAAAAACCACTTTTGCATATTTATTTTGTCATTATGTTTTGGCATTAATATGCTCAGCCAGAAGCTGGAAGCTACTTTTCCTGCTGTGCGTTTACTCGTATGTATGTCCTTGGTATTCATATAATTTGAATGACTTTT