CR80118

CRIMIC CR80118

Gene Nep1

CG5905

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFPPT_p1	X:6091205..6103971	5D4-5D5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	L-F AGCGACTGGCTGCTCAAG L-R gtgcagatgaacttcagggtcag	R-R TCACACCTACCTATGCGTAGATTAC R-F acgaagcgcctctatttatactcc

Images

Construct Design Sequences

sgRNA sequence	TAGCCCAACTACATAGTATCTGG
sgRNA2 sequence

left_200bp	right_200bp
CGCAGCAGCGGCGCCGCCTGCTCGTCCTGGCCATCGCCTTTACCGTTCTGGGCGCGGCCATCGGGGCACTGGCCATCTACTTCGCCAGCGTCCATCAGCGTTGCCATCTGTATCGCCTGGAGCCAGGTAAGGGTTAAGGTCGTGAAATACAATATATACATATGTATAACTAAGGTTCAGCCACATCGAAGATACCAGAT	ACTATGTAGTTGGGCTATGCCGAAATAGAGATCTAATTCACTTCGACAGACAGAAGTTTGTATTTTTTTTACTTATCAGTTTAAATTCCATTGTCCCACGATTTACTACCATTCTATCGAAAGCTATGTGCTGCTGTTTGATTGTGGTTTTCATGGCGAAACTTCCCGTTTTTATGGTGGAATTTTGAAAGCCCCAGAAT