CR80119

CRIMIC CR80119

Gene Nep3

CG9565

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFPPT_p0	X:19961297..19969323	19A3-19A3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	L-F CTTCGGTCTACTTATCAGATGCCC L-R gtgcagatgaacttcagggtcag	R-R ACGAACCGCCTCTCAAAGC R-F acgaagcgcctctatttatactcc

Images

Construct Design Sequences

sgRNA sequence	GAAGCCCAACGACAACTAAGTGG
sgRNA2 sequence

left_200bp	right_200bp
TCGATTCTCATTCCAGATGACGCGCTACAAGCAGACCGAATTCACGGAGGACGACTCGAGTTCCATCGGCGGCATTCAATTGAACGAAGCGACCGGCCATACGGGCATGCAGATACGCTACCACACCGCCCGGGTATGTAAGAAACCTGTCGGGGTAACACCGTGCATCCGTGCAATGCAATTCAGTCGGATAGCCACTT	AGTTGTCGTTGGGCTTCAGCTTTATCTTTGTGGCAATGACCCAGAAACGAGTGGCAAATGGGGCCAAAGTGAGCGCTTTTCTCATTTGCTCGAAGGTTGCCAGTTGACAGTGAAAAGTCTGCTCTTATGAGTGGGAGCGCGTTGGTGTGAGCTTTTCCCTCTGCGAACTGACATGAATACCCTATCTGGTGACAGGGTAA