CR80121

CRIMIC CR80121

Gene NetB

CG10521

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFPPT_p1	X:14686038..1749376	12F2-12F3

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	L-F ACACATTTTGAATAGCAAATTTATGGGGATC L-R gtgcagatgaacttcagggtcag	R-R ACATTTCGGTGGGTCCAAATAAC R-F acgaagcgcctctatttatactcc

Images

Construct Design Sequences

sgRNA sequence	GCAGGAGAAGATGGCGCACGCGG
sgRNA2 sequence

left_200bp	right_200bp
CGACTATGCCGATGATAAAGCATGAATTGATTGAGTAGCACATATTCTTTCATTGAAACCGAATTGTGGAACTGCCATTGATATTGATCTTTGGAGCTGCCCTCGGTCAAGAGCAGAAGGTCTTTTCATGGTCACTCACAGTGGACTCGGAATTGGACATTGGTGCGATTGAGTGCATTTGTTGCAGGAGAAGATGGCGC	ACGCGGTGGAGGTGGATGGCTGAAAAGTTGGGGACGGCGATTCGAGTGGCGTTTTCAATTGCGAGCATTGTGTGATTGCGATTGTGCTTGTGCCAGTGCCCTATGCTCATCCTTTCATCATCACTCCAGTTTCGCATTGCACTTGAGTACTTTAGCCTTGCACTGGCAATCGCCATCGATGCCATCGCCTCCACAGCTCC