CR80132

CRIMIC CR80132

Gene CG33090

CG33090

Information

Donor	Location	Cytology	FBti	Stock number
gRNA_int200_GFP_exon	II:14334158..14345795	35A4-35A4		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	L-F ccaatggcttcatagccaatgc L-R cccttggacaccatggatc	R-R gtacttcaacagagagtagcagtttgatttg R-F cgcatgattatctttaacgtacgtcac

Images

Construct Design Sequences

sgRNA sequence	GTCTTGTCCCTGGTGCTGGATGG
sgRNA2 sequence	ATCTTTGGTTGTGTAGACTTCGG

left_200bp	right_200bp
CAACGATTCTGGGTTATTTTGGGTTATCTGGCGCGACAACAATGATTTTGTAACGATATGGTATATATATGGTATATATGGTATGGTATGATGGACGTTTTACCTTGGTTTTCTTTCTTATGCACTTTGGTTTCGATTTGGTTTTGCTTTATTGCTTTGCCACTACCGCGCAATCACACACACCCACACCATGTCCATCC	TCTACACAACCAAAGATATTTCTAAGCCAGCACAAAGATTTTCTTAGCGTTTAGTTCGATTCTTCTTTTGAGGCTGAATGTGTAACGAGCTTATCGAGATGGTGCGAGTCGAGGAAGATGTTGTCTCTGAAGTGGTTTTCAAATTTATAGCTTTATCTGTACTTTTAAGCTGTGGAATCTGTTGGCGCTTACTCCATGTC