| Donor | Location | Cytology | FBti | Stock number |
|---|---|---|---|---|
| PM37-p2 | 2R:14246966..14252597 | 50E6 | FBti0220970 | Order from Bellen Lab |
PCR check relative to sgRNA sequence
| HA-L | HA-R | Forward | Reverse |
|---|---|---|---|
| short and weak band | OK | TCCTTCGCGAGTCATTCAGGTCAA | TCAAGATCTTGGCAGCAACCACAC |
 |
| sgRNA sequence | AAGCGTGGTCATTGTGTTATTGG |
| sgRNA2 sequence |